WHAT IS CHLOROPHYLL??!!??
As you know, chlorophyll is the green pigment located in thylakoid discs which is contained in chloroplast.This captures the light energy, combines it with carbon dioxide and water and turns it into glucose. But what and how does it contain?
As you know, chlorophyll is the green pigment located in thylakoid discs which is contained in chloroplast.This captures the light energy, combines it with carbon dioxide and water and turns it into glucose. But what and how does it contain?
Materials you would need:
-Chromatography paper
-A Pencil
-A leaf (Prefably Dark Green)
-A cork
-A test tube
-Water
-Aluminum foil
- Solvent - Ethanol, Isopropanol, Acetone, Hexan
1.) Firstly, boil water and pour it into a cup. Leave the leaf for about 5 minutes.Dry them.
Boiling water kills the cells and destroys enzymes which can promote chlorophyll degradation. It also breaks chloroplasts which makes chlorophyll extraction easier.
2.) Cut the veins and grind the rest up till it is consistent and able to flow freely.Spread it on an aluminum foil evenly and bake in the oven at 40 degree Celsius.
3.)Grind it in a mortar till it is of a uniform yellowish green colour. Put it in a test tube and add a few ml of solvent, close the lid and shake it.
4.) There should be emerald-green slightly opalescent liquid on top of dark green powder (which will sink to the bottom of the test tube)Right now it's diluted and solution contaminated with fine debris from the broken cells. Remains of chloroplasts and other cell components are floating in liquid making it slightly foggy.
To wrap this nicely...
we do SEDIMENTATION OF LEAF CELL DEBRIS!:D
-leave the solution to sit after 10minutes and let the powder sit, pour the liquid out EXTREMELY carefully.
-Pour the top 90% into a third test tube. Leave it in a well ventilated place for a few days and check it from time to time. You would be able to see the different colours and layers in it
AND YOU'RE DONE!(:
AND YOU ARE DONE!!!!:D
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